Tuesday, February 18, 2020

Data analysis Essay Example | Topics and Well Written Essays - 500 words

Data analysis - Essay Example The Experiment of Western blotting analysis for the samples of NIH/3T3 cells, whether having been treated in PDGF or not, uses the Phospho-Akt (Ser473) for the preparation of the upper blotting and Akt 9272 for the lower blotting. It has to present the results in a tabular form, the first column being PGDF, the second being AKT and the third column being Phospho-Akt. This was a time series practical activity to stimulate the cells at various durations from 0 to 120 minutes. Its independent variable was the PGDF. There are two blots to analyze, Ser473 of Akt as the upper blot (using phospho-Akt antibody) and phosphor-Akt (using Akt antibody) as the lower blot and. It also involved using gels to conduct the molecular weight marker. The top band corresponded to the measurement of 76 kDa, while the middle band corresponded to 57 kDa. The lowest band thus corresponded to 47 kDa. The growth factor analysis shows that proteins facilitate growth at different rates under different conditions. The most relevant protein analysis procedure in this kind of experiment is the florescent microscopy. From the principles of cytometry, this experiment conducts the flow cytometry experiment with the labeling of Jurkat cells and the labeling of Phospho-Akt ser 473antibody. It uses three categories of cells, untreated, treated cells and control experiment (Burgering and Coffer 1995, p54). All the cells are labeled in three different colors as they appear in the graph above. The red line is the control experiment, green is treated and blue is

Monday, February 3, 2020

Disability and Discrimination Research Paper Example | Topics and Well Written Essays - 2000 words

Disability and Discrimination - Research Paper Example lity of people with disabilities whether their disability is artificial or natural and whether we are required to equalize people because of their disabilities (p.15). Though world has recognized the rights of differently-abled persons, little has been done to see the compliance. Disability Discrimination is the process of making decisions affecting an employee based wholly or partly, upon the real or perceived disability of the employee (Davidovich, 2003). According to Brading and Curtis (2000) many employers fail to appreciate that disability does not necessarily imply ability and a disability may not result in handicap therefore assuming that the people with disabilities would not be able to perform adequately at work (p.16). There are several practices of discrimination going on against person with disabilities and it is assumed that in spite of various acts and legislations passed by national and international bodies, the barrier and stigma still exists in the 21st century as co ntemplated by Brading andCurtis (2000). This paper, therefore, makes in depth analysis regarding discriminatory practices against person with disabilities to the extent of discriminatory practices. Statement of the Problem As stated above, there are several practices of discrimination going on against person with disabilities, but the major form of discrimination comes alive at workplace. Despite Disability Discrimination Act 1995 being in force, making unlawful for an employer for treating disabled person less favourable, the number of discriminatory practice is soaring (Brading and Curtis, 2000, p.15). Why? Why are such discriminations taking place and whether enacted laws and legislations are proving effective? Review of Literature According to International Classification of Functioning... This reprot approves that tThere are several practices of discrimination going on against person with disabilities and it is assumed that in spite of various acts and legislations passed by national and international bodies, the barrier and stigma still exists in the 21st century as contemplated by Brading and Curtis. This paper, therefore, makes in depth analysis regarding discriminatory practices against person with disabilities to the extent of discriminatory practices. According to International Classification of Functioning disability arises when an individual’s ability to do daily activities, carry out social roles and participate in communal activities that are considered normal or common by the individual and the community, is limited or hindered because of a bodily, sensory, mental and/or intellectual function and environmental barriers. This paper makes a conclusion that discrimination on people with disability is still prevalent in high economy countries and the apathy of these people in developing and underdeveloped countries can be better understood in the words of Mahmud and Nahala in Yemen. The researches carried out by different agencies show approximate status and scenario of opportunities available to the people with disabilities even in 21st century. Though laws and legislations have been enacted by the International bodies including WHO, ILO and United Nations prohibiting defiance of rights to persons on the basis of race, color, religion, creed, place, and disability among others, the gap remains in the rate of employment and unemployment as well in wages.